Molecular mechanisms of abnormal chromosome segregation caused by genomic shock.

2017 Fiscal Year Final Research Report

• Project/Area Number: 25450515
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Osaka Kyoiku University
• Principal Investigator: Suzuki Go 大阪教育大学, 教育学部, 教授 (10314444)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Keywords: 染色体工学 / ソマクローナル変異 / 混数性 / タバコ / 細胞分裂

Outline of Final Research Achievements

Mixoploidy is the phenomenon whose cells maintain different karyotypes in one plant. In tobacco plants, genetically-regulated mixoploidy is probably caused by abnormal chromosome segregation triggered by genomic shock. In the present study, we performed molecular genetic experiments about tobacco mixoploidy using our original transgenic lines by immunohistochemical analysis, transcriptome analysis with methylated DNA assay, and functional assay with transgenic plants. From this study, we obtained important information about "dynamic chromosome behavior" and "molecular basis of somaclonal variations".

Free Research Field

植物分子遺伝学