2015 Fiscal Year Final Research Report
Mitosis regulation by Apollon
| Project/Area Number |
25460084
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
Naito Mikihiko 国立医薬品食品衛生研究所, 遺伝子医薬部, 部長 (00198011)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | サイクリンA / Apollon / 細胞周期 / ユビキチン |
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| Outline of Final Research Achievements |
During mitosis, cyclin A and cyclin B are ubiquitylated by the anaphase promoting complex/cyclosome (APC/C) and then subjected to proteasomal degradation. However, cyclin A, but not cyclin B, begins to be degraded in the prometaphase when APC/C is inactivated by the spindle assembly checkpoint (SAC). Here we show that Apollon (also known as BRUCE or BIRC6) plays a role in SAC-independent degradation of cyclin A in early mitosis. Apollon interacts with cyclin A and facilitates cyclin A ubiquitylation. In Apollon-deficient cells, mitotic degradation of cyclin A is delayed, and total but not CDK-bound cyclin A level was increased. We propose Apollon to be a novel regulator of mitotic cyclin A degradation independent on SAC.
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| Free Research Field |
細胞生物学
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