2015 Fiscal Year Final Research Report
Metabolism of major phytocannabinoids in human brain and lung microsomes and its toxicological implication
| Project/Area Number |
25460176
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Hokuriku University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 大麻成分 / テトラヒドロカンナビノール / カンナビジオール / カンナビノール / 脳 / 肺 / 代謝 / 細胞毒性 |
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| Outline of Final Research Achievements |
Metabolic interactions of major phytocannabinoids (tetrahydrocannabinol, THC; cannabidiol, CBD; cannabinol, CBN) with microsomes from human brain, lung and placenta were investigated as part of toxicological study of these cannabinoids on extrahepatic tissues and following points were clarified: (1 & 2) metabolism of cannabinoids with human brain and lung microsomes. (3) metabolism of cannabinoids with human placenta microsomes and CYP19. (4) microsomal aldehyde oxygenase (MALDO) activity in human brain and lung microsomes for 11-oxo-THC. (5) inhibitory effects of cannabinoids on hydrolysis of anandamide and 2-arachinoylglycerol with human brain and lung microsomes. (6) Cytotoxicity of THC metabolites to mouse lung macrophage J774-1 cells.
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| Free Research Field |
衛生化学裁判化学
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