2015 Fiscal Year Final Research Report
The role of cytosolic ATP in VSOR channel activity
| Project/Area Number |
25460304
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 調節性容積減少(RVD) / ABCF2 / アクチニン-4 / 容積感受性アニオンチャネル(VSOR) / 細胞内ATP |
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| Outline of Final Research Achievements |
Volume regulation is fundamental mechanism for animal cells. By the exposure to hypotonic stimulation, cells show the regulatory volume decrease (RVD) to efflux osmotically obligated water, which is accomplished by the efflux of K+ and Cl-. A volume-sensitive outwardly rectifying Cl- channel (VSOR) plays a major role in RVD, and it shows cytosolic ATP dependence. Recently, we reported that a swelling-enhanced interaction between α-actinin-4 (ACTN4) and ABCF2 prevents ABCF2 from suppressing VSOR activity and results in the facilitation of RVD. ABCF2 has nucleotide binding domains. So, we studied the possibility that ABCF2 is involved in the requirement of cytosolic ATP for the VSOR activity. With point mutants of ABCF2, we investigated their affinity to ATP and ACTN4 and found that ABCF2 with higher affinity to ATP has higher ability to bind with ACTN4. From these results, we considered cytosolic ATP effects on the interaction between ACTN4 and ABCF2 and modifies VSOR channel activity.
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| Free Research Field |
分子細胞生理学
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