2015 Fiscal Year Final Research Report
Activation mechanisms of AMPK by nitrite
| Project/Area Number |
25460332
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAMAKI Toshiaki 徳島大学, 大学院医歯薬学研究部, 教授 (80179879)
IKEDA Yasumasa 徳島大学, 大学院医歯薬学研究部, 准教授 (60432754)
KIHIRA Yoshitaka 徳島大学, 大学院医歯薬学研究部, 助教 (90377276)
ISHIZAWA Keisuke 徳島大学, 大学院医歯薬学研究部, 教授 (60398013)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 亜硝酸塩 / AMPK |
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| Outline of Final Research Achievements |
We have shown that dietary supplied nitrate, which substantially exists in our daily diets including vegetables, is reduced into nitrite, and becomes a significant source for NO production playing tissues- and vaso-protective roles especially in hypoxic tissues. During the study period, we found a phenomenon that nitrite directly activated the AMP-activated protein kinase (AMPK) in a concentration dependent manner. To clarify this mechanism, we investigated the activation of AMPK by a549 cells that lacks LKB1 and fails to facilitate the AMPK phosphorylation. In addition, pre-treatment of STO609, a CaMKK inhibitor, suppressed the AMPK phosphorylation. These data suggest that nitrite activates AMPK through both LKB1 and CaMKK pathway.
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| Free Research Field |
医歯薬学
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