Study of lung cancer cell invasion mechanism through PtdIns imaging

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460433
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Akashi Takumi 東京医科歯科大学, 医学部附属病院, 准教授 (60242202)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 肺癌 / 浸潤 / アクチン / ホスファチジルイノシトール / 基底膜

Outline of Final Research Achievements

Alterations in subcellular localization of cortical actin in lung adenocarcinomas and its correlation with metastasis and poor prognosis was clarified by histological examination of surgically resected tissues. In 3D-cultured lung cancer A549 cell spheroids, accumulation of PtdIns(4,5)P2 monitored by GFP-tagged PH-PLCD probe and phosphorylated myosin light chain as well as F-actin were observed. The basement membrane was considered to be one of the regulator of cortical actin accumulation through phosphorylated myosin-mediated contraction and PtdIns(4,5)P2-mediated F-actin assembly. Myosin inhibitor Blebbistatin suppressed 3D collective migration of A549 cells induced by constitutively active Cdc42 and MT1-MMP which suggests the participation of matrix-side F-actin in invasion through myosin-mediated contraction.

Free Research Field

人体病理学