2015 Fiscal Year Final Research Report
Screening of inhibitors against influenza A virus PA endonuclease
| Project/Area Number |
25460574
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | インフルエンザ / ウイルス / RNA ポリメラーゼ / エンドヌクレアーゼ / PA / フラーレン / 漢方 |
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| Outline of Final Research Achievements |
To identify potential new anti-influenza compounds, we screened 12 fullerene derivatives using an in vitro influenza A RNA polymerase PA endonuclease inhibition assay. We identified 8 fullerene derivatives that inhibited the endonuclease activity of the PA N-terminal domain or full-length PA protein in vitro. In a cell culture system, we found that several fullerene derivatives inhibit influenza A viral infection and the expression of influenza A nucleoprotein and nonstructural protein 1. These results indicate that fullerene derivatives are possible candidates for the development of novel anti-influenza drugs.
Furthermore, the Kampo medicines kakkonto, shosaikoto, saikokeishito, keishito, maobushisaishinto, and maoto, but not chikujountanto, inhibited PA endonuclease activity in a dose-dependent manner. Our results indicate that Kampo medicines are good sources providing a structural lead for optimization of an influenza endonuclease inhibitor.
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| Free Research Field |
生化学
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