Identification of small compounds which are associated with NS4A and inhibit the growth of dengue viruses.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460577
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Tajima Shigeru 国立感染症研究所, その他部局等, 主任研究官 (60311346)
• Research Collaborator: TOHMA Daiki
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: デングウイルス / 抗ウイルス薬 / 非構造蛋白質 / サイクロフェニル

Outline of Final Research Achievements

Dengue virus (DENV) is the etiologic agent responsible for dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. The infections have emerged and continue to spread rapidly. Now no effective antiviral drugs exist to treat DENV infection and licensed vaccine is only available in a few countries.　We show here that a compound cyclofenil (CF) has an inhibitory effect on growth of DENV in mammalian cells in vitro. CF exhibited antiviral activity to DENV in mammalian cells but not in mosquito cells, suggesting that CF may interact with a mammalian cell factor. Time-of-addition experiment indicates that CF may inhibit the late stage of the life-cycle of the DENV. We also succeeded in isolating CF-resistant virus, and several mutations with amino acid substitutions were identified in the mutant virus genome.

Free Research Field

医歯薬学