Development of high sensitivity antibody protein chip using tagged antibody produced by genetic recombination technology.

2016 Fiscal Year Final Research Report

• Project/Area Number: 25460689
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Yamaguchi University
• Principal Investigator: FURUMOTO Hiroko 山口大学, 医学(系)研究科(研究院), 講師 (70311818)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 蛋白チップ

Outline of Final Research Achievements

An antibody chip is a tool used to exhaustively analyze protein expression in full detail. The purpose of this study is to create a new technology for antibody arrays by developing a highly sensitive next generation antibody chip, using the technology where a tagged protein is immobilized on a diamond-like surface treated substrate. Thus, we created a monoclonal antibody where the tag-sequence was added to immunoglobulin G (IgG) using genetic recombination technology. The tag introduced IgG and a control IgG were produced in a cell culture system. Then, they were fixed to the substrate to evaluate the state of the antibody and antigen-detection efficiency on the substrate with or without the tag. As a result, it was confirmed that a tagged-antibodies are high sensitivity, but hundreds of different kind of tagged-antibodies are required for the creation of the antibody array. Therefore, a high-throughput production system of the tagged-antibody, such as genome editing is under trial.

Free Research Field

生化学