Establishment of biological dose assessment method using the chromosome analysis in low-dose radiation exposure.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460694
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Fukushima Medical University
• Principal Investigator: Sakai Akira 福島県立医科大学, 医学部, 教授 (70284221)
• Co-Investigator(Kenkyū-buntansha): KATAFUCHI Atsushi 福島県立医科大学, 放射線生命科学講座, 助教 (40634910) ; OGAWA Kazuei 福島県立医科大学, 循環器血液内科, 教授 (40423800) ; YOSHIDA Mitsuaki 弘前大学, 被ばく医療総合研究所, 教授 (60182789)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 低線量被ばく / 二動原体染色体 / 転座型染色体 / 医療被ばく / CT検査

Outline of Final Research Achievements

We performed dicentric chromosome assays (DCAs) and translocation analyses (chromosome painting) before and after a CT scan to assess effects of low-dose ionizing radiation on chromosomes. DCA was performed by analyzing either 1,000 or 2,000 metaphases using both Giemsa staining and centromere-fluorescence in situ hybridization (Centromere-FISH). Translocation analysis was performed by analyzing 2,000 cell equivalents. DIC formation increased significantly after a CT scanning. A good correlation was observed between the increment of DIC formation after a CT scan determined by analysis of 1,000 metaphases and those by 2,000 metaphases using Centromere-FISH, and between the increment of DIC formation determined by analysis of 2,000 metaphases using Giemsa staining and those by 2,000 metaphases using Centromere-FISH. In contrast to the results of DIC formation, the frequency of translocations detected before and after the CT scan did not differ significantly.

Free Research Field

放射線生物学