2015 Fiscal Year Final Research Report
The development of novel, quick, and simple FRET-based telomerase assay method
Project/Area Number |
25460708
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory medicine
|
Research Institution | Konan University |
Principal Investigator |
MURASHIMA TAKASHI 甲南大学, フロンティアサイエンス学部, 教授 (20263923)
|
Co-Investigator(Renkei-kenkyūsha) |
MIYOSHI Daisuke 甲南大学, フロンティアサイエンス学部, 教授 (50388758)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | 凝集誘起発光 / テロメア / アミロイドβ / ガン / アルツハイマー病 |
Outline of Final Research Achievements |
A simple and sensitive methods for measuring telomeric tandem repeat DNA and amyloid-β based on AIE phenomena were developed. According to these methods, telomere concentration can be estimated with high sensitivity (5 pM for 12-mer telomeric sequences) using AIE-CCCTAACCCTAA probe. On the other hand, the detection of Aβ was performed with AIE dye-amyloid fibrillization-promoting peptide (AFPP) probe. While Aβ was added to the probe solution, the aggregation of Aβ was promoted and the fluorescence was enhanced within 3 hrs. The limit of detection with this method is 4.2 nM. This method provides a simple, rapid, sensitive and cost-effective assay for telomere sequence and Aβ.
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Free Research Field |
有機合成化学
|