We have succeeded in controlling seizures by the transplantation of neural stem cells to the dorsal hippocampus of epileptic mutant EL mice.

2016 Fiscal Year Final Research Report

• Project/Area Number: 25461738
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: Murashima Yoshiya 首都大学東京, 人間健康科学研究科, 客員研究員 (50182118)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 神経幹細胞 / 移植 / 抑制性神経系 / 神経可塑性 / 抗てんかん機構

Outline of Final Research Achievements

Pluripotent embryonic stem (ES) cells may differentiate into neurons in vitro. It has been shown that astrocyte-derived factors promote mouse ES cells to differentiate into neurons. Cultured in astrocyte-conditioned medium (ACM) under free-floating conditions, within 4 days, colonies of undifferentiated mouse ES cells give rise to floating spheres of concentric stratiform structure with a periphery of neural stem cells, which are termed NSS. Culturing the spheres on an adhesive substrate in ACM promotes neurogenesis, and cells in the spheres differentiate intoneurons within 5 days . The mechanism was examined how neural stem cells (NSCs) transplanted to the hippocampus of EL brains differentiate into inhibitory neurons and play a role of preventing seizures. that brain regions involved in seizure formation, such as hippocampus and parietal cortex in EL mice, have a potency to control seizures by inducing inhibitory GAD neurons.

Free Research Field

神経可塑性