Isolation of primary culture of human breast cancer cells and analysis of molecular mechanism of tumor progression and metastasis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461972
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: University of Tsukuba
• Principal Investigator: Bando Hiroko 筑波大学, 医学医療系, 准教授 (00400680)
• Co-Investigator(Kenkyū-buntansha): Ohneda Osamu 筑波大学, 医学医療系, 教授 (30311872)
• Research Collaborator: Tachi Kana 筑波大学, 大学院, 博士課程 ; Shiraishi Akira 筑波大学, 大学院, 博士課程
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 乳癌 / 腫瘍幹細胞 / エストロゲン受容体 / Luminal type / FOXA1 / 低酸素 / HIF1 / ホルモン治療抵抗性

Outline of Final Research Achievements

It has been reported that ER‐positive and HER2‐negative breast cacer patients, so‐called luminal type breast cancer, can lead to late recurrence compared to other BC subtypes, possibly due to the presence of dormant tumor cells. The mechanisms that maintain cancer stem cells (CSCs) in luminal breast cancer remains obscure. We established luminal breast cancer cells derived from patient tissue and analyzed the potency of CSC and related factors with established luminal breast cancer cell lines. Our findings showed that the CSC population in luminal breast cancer induces ectopic expression of FOXA1. The expression of FOXA1 appears to be involved in the proliferation of immature breast cancer cells. Hypoxic stimuli promote the induction of ALDH activity, suggesting the function of hypoxia on tumor environment may influence BCSC fate.

Free Research Field

乳腺腫瘍学