Mutanome analyses by next generation sequencing and individualized cancer vaccine development in gastric cancer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462014
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: The University of Tokyo
• Principal Investigator: Wada Ikuo 東京大学, 医学部附属病院, 届出診療医 (80401082)
• Co-Investigator(Kenkyū-buntansha): SETO Yasuyuki 東京大学, 医学部附属病院, 教授 (00260498) ; KAKIMI Kazuhiro 東京大学, 医学部附属病院, 特任教授 (80273358) ; MATSUSHITA Hirokazu 東京大学, 医学部附属病院, 特任講師 (80597782)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 胃がん / 次世代シーケンス / 遺伝子変異 / MHCクラスI結合予測法 / 固有抗原 / TIL / 個別化がん免疫治療

Outline of Final Research Achievements

We established the method to identify unique antigens from cancer specific mutations using murine gastric cancer model by combining next generation sequencing with MHC class I binding algorism. We obtained cancer tissues and peripheral blood mononuclear cells (PBMCs) from 29 gastric cancer patients. We analyzed the expression of immune checkpoint molecules and the infiltration of regulatory T cells (Tregs) to understand the immune suppressive tumor microenvironment in 10 patients. We successfully cultured tumor infiltrating lymphocytes (TILs) specifically recognizing autologous tumor cells from 3 patients. We created the system predicting candidate unique antigens and obtained promising samples including cancer tissues and TILs, which are a prerequisite for individualized cancer immunotherapy development in gastric cancer patients.

Free Research Field

消化器外科学