2015 Fiscal Year Final Research Report
Development of therapeutic method for heart failure through cellular phenotype control using susutained release of microRNA
Project/Area Number |
25462136
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cardiovascular surgery
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Research Institution | Kyoto University |
Principal Investigator |
Yamazaki Kazuhiro 京都大学, 医学(系)研究科(研究院), 助教 (50464227)
|
Co-Investigator(Kenkyū-buntansha) |
ONO KOH 京都大学, 大学院医学研究科, 准教授 (00359275)
SAKATA RYUZO 京都大学, 大学院医学研究科, 名誉教授 (20325781)
TABATA YASUHIKO 京都大学, 再生医科学研究所, 教授 (50211371)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | 心筋疾患外科学 / 心筋リモデリング / 心不全 |
Outline of Final Research Achievements |
We performed the evaluation for optimal mixture condition of gelatin hydrogel and miR-29b using COS-7 cells. We could found the condition to efficiently induce miR-29b complementary plasmid within COS-7, providing suitable condition of better microRNA induction using sustained-release vehicles. We next evaluated the efficiency of transduction of miR-126-incorporated poly(lactic-co-glycolic acid)(PLGA) for mice hindlimb ischemia model. Treatment with miR-126-incorporated PLGA led to increased blood flow and activation of signaling pathway related to miR-126 in gene and protein expression level. We showed fair efficiency of transduction of microRNA into the target cells which provides a basic knowledge for sustained release of microRNA into the heart muscle tissue.
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Free Research Field |
心臓血管外科学
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