2015 Fiscal Year Final Research Report
Development of a sensitive system for capturing circulating tumor cells in lung cancer.
| Project/Area Number |
25462191
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory surgery
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
SO Tomoko 産業医科大学, 医学部, 講師 (00341529)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Fumihiro 産業医科大学, 医学部, 教授 (10283673)
URAMOTO Hidetaka 産業医科大学, 医学部, 准教授 (90389445)
CHIKAISHI Yasuhiro 産業医科大学, 医学部, 助教 (25861251)
YONEDA Kazue 産業医科大学, 医学部, 助教 (80724806)
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| Research Collaborator |
KATAYORI Satoshi JSRライフサイエンス株式会社, 研究開発部
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 肺癌 / 循環腫瘍細胞 |
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| Outline of Final Research Achievements |
The “CellSearch” is widely used to detect circulating tumor cells (CTCs), but provided only modest sensitivity in capturing CTCs in lung cancer patients. In the current study, we employed a variety of CTC-detection systems including a novel microfluidic device (“CTC-chip”) in which any antibody to capture CTCs is easily conjugated. In experimental studies using tumor cell lines, the “CTC-chip” provided a similar efficiency to the “CellSearch” in capturing PC-9 cells with EpCAM expression. When MESO-4 cells without EpCAM expression were spiked in the blood, approximately 80% of tumor cells were captured with the “CTC-chip”, whereas few tumor cells were captured with the “CellSearch”.
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| Free Research Field |
呼吸器外科学
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