2016 Fiscal Year Final Research Report
Analysis of core fibril-forming collagen gene regulation in bone for appication of regenerative medicine
| Project/Area Number |
25462375
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 骨代謝 / コラーゲン / 遺伝子発現 / 転写因子 / マイクロRNA / 再生医療 |
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| Outline of Final Research Achievements |
The extracellular mtrix of bone is composed of collagen, primary type I collagen with other type of collagen such as type V and type XII collagen. In the present study, we examined whether specific transcription factor regulates the pro-a2(I) collagen gene (Col1a2) in bone. Sp7/Osterix induced the Col1a2 gene expression via the proximal promoter in osteoblastic cells. MicroRNA (miR)s regulate gene expression by binding to the 3'-untranslated region (3'UTR) of specific targent mRNA. We examined the regulation of a1(V) collagen gene (Col5a1) expression by miRs in culture cells. miR-29 is involved in the down-regulation of Col5a1 gene expression in osteoblastic cells and fibroblasts. The results suggest that these factors might be applied for regenerative medicine such as therapy of osteoporosis.
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| Free Research Field |
分子細胞生物学
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