Effects of local Renin-angiotensin system on development and progression of knee osteoarthritis(OA)

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462389
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kinki University
• Principal Investigator: AKAGI Masao 近畿大学, 医学部, 教授 (00273441)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 変形性膝関節症 / レニン・アンジオテンシン系 / つくば高血圧マウス / 骨粗鬆症

Outline of Final Research Achievements

C57/BL6 mice and Tsukuba hypertensive mice (THM) were forced to run on a treadmill. The lateral compartments of the left knees were evaluated histopathologically using the OARSI score. Additionally, expressions of AT1R, AT2R, ACE1, and angiotensinogen were evaluated immunohistologically, after 8 weeks of running. In the THM, expressions of AT1R and AT2R in chondrocytes were evaluated each week, to clarify when AT1R and AT2R were expressed and involved in cartilage degeneration. The mean OARSI scores of the lateral knee compartment were significantly higher in the THM than in the C57BL/6 mice after 4, 6, and 8 weeks (P<0.05). Expressions of RAS components were detected in the THM but not in the C57BL/6 mice after 8 weeks. In the THM, expression of AT1R and AT2R were detected after 2, 4, 6, and 8 weeks, but not at 0 weeks. Activation of the RAS could accelerate expression of AT2R on chondrocytes and murine knee OA developed which mechanical stress of the forced running.

Free Research Field

外科系臨床医学・整形外科学