Effect of nanofiber PGA in composite scaffolds with polypropylene for fabricating tissue engineered auricle

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462806
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Kinki University
• Principal Investigator: ISOGAI Noritaka 近畿大学, 医学部, 教授 (90203067)
• Co-Investigator(Kenkyū-buntansha): KUSUHARA Hirohisa 近畿大学, 医学部, 講師 (50388550) ; MOROTOMI Tadaaki 近畿大学, 医学部, 講師 (10388580)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: PGA / ナノファイバー / 耳介軟骨 / 再生誘導 / 自家移植

Outline of Final Research Achievements

In conventional studies on the regeneration of auricle-shaped cartilage in autogenous models using large animals, there were problems with the cartilage regeneration induction capacity and long-term retention of geometric shape. In this study, we sought to improve upon outcome in these regards: a non-woven fabric of polyglycolic acid (PGA) was developed through nanotechnology, evaluating the effect of nanofiber diameter on in vitro cell-seeding efficiency, and in vivo response after implantation in an autogenous large-animal model. Optimal cell maintenance and neocartilage response were seen with PGA fiber diameters of 0.8-3.0 μm for nano-fiber constructs. Biomechanical strength was optimal for PGA fiber diametersin in the same mid-range. These findings demonstrate the potential for nano-scale modulation of auricle-shaped cartilage regeneration in a large-animal model.

Free Research Field

医歯薬学