A development of the bonding treatment of vertically fractured roots with periodontal regeneration on adhesive resin using nanotechnology

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462943
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Hokkaido University
• Principal Investigator: TANAKA SAORI 北海道大学, 大学病院, 講師 (90344522)
• Co-Investigator(Kenkyū-buntansha): MIYAJI HIROFUMI 北海道大学, 北海道大学病院, 講師 (50372256) ; INOUE KANA 北海道大学, 北海道大学病院, 医員 (40600127) ; TANAKA TORU 北海道大学, 北海道大学病院, 講師 (90179771)
• Co-Investigator(Renkei-kenkyūsha): TANINO MISHIE 北海道大学, 医学研究科, 講師 (90360908) ; KATO AKIHITO 北海道大学, 歯学研究科, 助教 (40507571)
• Research Collaborator: KANAYAMA IZUMI 北海道大学, 歯学研究科, 大学院生 ; NISHIDA ERIKA 北海道大学, 歯学研究科, 大学院生 ; MURAKMI SHUSUKE 北海道大学, 歯学研究科, 大学院生 ; KAWAMOTO KOHEI 北海道大学, 歯学研究科, 大学院生 ; MIYATA SAORI 北海道大学, 歯学研究科, 大学院生
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ナノテクノロジー / 歯周組織再生 / カーボンナノチューブ / ナノβ-TCP / 細胞培養シート / レジン

Outline of Final Research Achievements

Composite resin (CR) is used the bonding treatment of vertically fractured roots and as a retrograde material following apiccectomy. We aimed to the periodontal regeneration on CR. Effective biological modification of the CR surface is thus needed to improve its biocompatibility. Carbon nanotubes (CNT) and β-TCP have useful properties such as promoting cell adhesion and proliferation. CR discs were coated by immersion in a dispersion of CNT and β-TCP. In the cell culture assay, the CNT-CR samples exhibited abundant cells and marked adhesion of filopodia to CNT. Cell proliferation assay indicated that the CNT-CR was significantly higher than the CR . This results showed that coating the surface of CR with CNT improved their biocompatibility. In vivo studies, we observed the bone-like hard tissue formation on a CNT-CR wrapped in cultured bone marrow stromal cell sheets. Thus, this experiment might suggest a possibility of the periodontal regeneration on CR.

Free Research Field

歯内療法学