Basic research of tissue engineering for TMJ using immortalized TMJ disc cell clones

2015 Fiscal Year Final Research Report

• Project/Area Number: 25463170
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Hosomichi Jun 東京医科歯科大学, 医歯(薬)学総合研究科, 講師 (00420258)
• Co-Investigator(Kenkyū-buntansha): YONEMITSU Ikuo 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (00431940) ; SHIMIZU Yasuhiro 東京医科歯科大学, 歯学部附属病院, 医員 (60631968)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 顎骨低形成 / 骨軟骨破壊

Outline of Final Research Achievements

Our first purpose was to clarify the mechanism of osteoarthritis in TMJ, leading to disturb TMJ regeneration in adults, especially in female adults. We checked cell responses of TMJ disc clone cells to female hormone, relaxin. Relaxin enhanced the expression of RXFP1/2 in fibroblast-like clones. It caused a greater induction of MMP9/13 in fibroblast- than in chondrocyte-like clones. Our second purpose was to examine the role of intermittent hypoxia (IH) observed in pediatric obstructive sleep apnea (OSA) in dentofacial morphological changes in growing rats. We demonstrated that IH induces the formation of a smaller mandible and larger tongue in peripubertal rats, consistent with children with OSA who exhibit macroglossia and retrognathia, as well as discrepant growth in the transverse dental arch. Mandibular growth persists during adolescence, suggesting that IH induces earlier intramembranous ossification and growth delay in the mandible in growing rats.

Free Research Field

歯科矯正学