Role of clock genes in macrophages in the crosstalk between exercise and immunity

2013 Fiscal Year Final Research Report

• Project/Area Number: 25560340
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Sports science
• Research Institution: Kyorin University
• Principal Investigator: OHNO Hideki 杏林大学, 医学部, 教授 (00133819)
• Co-Investigator(Renkei-kenkyūsha): KIZAKI Takako 杏林大学, 医学部, 教授 (00322446) ; SAKURAI Takuya 杏林大学, 医学部, 講師 (20353477) ; OGASAWARA Junetsu 杏林大学, 医学部, 助教 (20415110)
• Project Period (FY): 2013-04-01 – 2014-03-31
• Keywords: マクロファージ / Rev-erbα / 単球走化性因子 / 炎症反応

Research Abstract

Overexpression of Rev-erb in murine macrophage cell line RAW264 cells suppressed the induction of Ccl2 following an LPS endotoxin challenge, resulting in the impaired activity of cell adhesion and migration. In addition, Rev-erb represses Ccl2 expression directly through a Rev-erb binding motif in the Ccl2 promoter region. These findings demonstrate that Rev-erb modulates the inflammatory function of macrophages through the direct regulation of Ccl2 expression.

Research Products

• [Journal Article] ETAS, an enzymetreated asparagus extract, attenuates amyloid β -induced cellular disorder in PC12 cells (2014)
  • Author(s): Ogasawara J, Ito T, Wakame K, Kitadate K, Sakurai T, Sato S, Ishibashi Y, Izawa T, Takahashi K, Ishida H, Takabatake I, Kizaki T & Ohno H
  • Journal Title: Nat Prod Commun Volume: 9 Pages: 561-564
  • Peer Reviewed
• [Journal Article] The effects of longterm exercise on cerebral function and the maintenance of concentration in the elderly (2014)
  • Author(s): Haga S, Sakurai T, Sato S, Sasahara M, Aita F, Esaki K, Toshinai K, Ueya E, Hashimoto N, Ogasawaea J, Kizaki T, Yoshinaga I, Sakurai T, Oh-ishi S, Ohno H & Takakuwa E
  • Journal Title: J Exer Sports Orthop Volume: 1 Pages: 6-11
  • Peer Reviewed
• [Journal Article] A circadian clock gene, Rev-erbα , modulates the inflammatory function of macrophages through the negative regulation of Ccl2 expression (2014)
  • Author(s): Sato S, Sakurai T, Ogasawara J, Takahashi M, Izawa T, Imaizumi K, Taniguchi N, Ohno H & Kizaki T
  • Journal Title: J Immunol Volume: 192 Pages: 407-417
  • DOI: 10.4049/jimmunol.1301982
  • Peer Reviewed
• [Journal Article] Enzyme-treated Asparagus officinalis extract shows neuroprotective effects and attenuates cognitive impairment in senescenceaccelerated mice (2014)
  • Author(s): Sakurai T, Ito T, Wakame K, Kitadate K, Arai T, Ogasawara J, Kizaki T, Sato S, Ishibashi Y, Fujiwara T, Akagawa K, Ishida H & Ohno H
  • Journal Title: Nat Prod Commun Volume: 9 Pages: 101-106
  • Peer Reviewed
• [Journal Article] Association of blood pressure and body mass index with intraocular pressure in middle-aged and older Japanese residents : a cross-sectional and longitudinal study (2014)
  • Author(s): Yoshida M, Ishikawa M, Karita K, Kokaze A, Harada M, Take S & Ohno H
  • Journal Title: Acta Med Okayama Volume: 68 Pages: 27-34
  • URL: http://www.lib.okayama-u.ac.jp/www/acta/pdf/68_1_27.pdf
  • Peer Reviewed
• [Journal Article] Intracellular β 2-adrenergic receptor signaling specificity in mouse skeletal muscle in response to single-dose β 2-agonist clenbuterol treatment and acute exercise (2013)
  • Author(s): Sato S, Shirato K, Mitsuhashi R, Inoue D, Kizaki T, Ohno H, Tachiyashiki K & Imaizumi K
  • Journal Title: J Physiol Sci Volume: 63 Pages: 211-218
  • DOI: 10.1007/s12576-013-0253-z
  • Peer Reviewed
• [Journal Article] Oxygen consumption and usage during physical exercise : the balance between oxidative stress and ROSdependent adaptive signaling (2013)
  • Author(s): Radak Z, Zhao Z, Koltai E, Ohno H & Atalay M
  • Journal Title: Antioxid Redox Signal Volume: 18 Pages: 1208-1246
  • DOI: 10.1089/ars.2011.4498
  • Peer Reviewed
• [Journal Article] Redox-regulating sirtuins in aging, caloric restriction, and exercise (2013)
  • Author(s): Radak Z, Koltai E, Taylor AW, Higuchi M, Kumagai S, Ohno H, Goto S & Boldogh I
  • Journal Title: Free Radic Biol Med Volume: 58 Pages: 87-97
  • DOI: 10.1016/j.freeradbiomed.2013.01.004
  • Peer Reviewed
• [Journal Article] Oligomerised lychee fruit-derived polyphenol attenuates cognitive impairment in senescence-accelerated mice and endoplasmic reticulum stress in neuronal cells (2013)
  • Author(s): Sakurai T, Kitadate K, Nishioka H, Fujii H, Ogasawara J, Kizaki T, Sato S, Fujiwara T, Akagawa K, Izawa T & Ohno H
  • Journal Title: Br J Nutr Volume: 110 Pages: 1549-1558
  • DOI: 10.1017/S000711451300086X
  • Peer Reviewed
• [Journal Article] Endogenous oxidative stress, but not ER stress, induces hypoxia-independent VEGF120 release through PI3K-dependent pathways in 3T3-L1 adipocytes (2013)
  • Author(s): Takahashi K, Miyokawa-Gorin K, Handa K, Kitahara A, Moriya R, Onuma H, Sumitani Y, Tanaka T, Katsuta H, Nishida S, Yoshimoto K, Ohno H & Ishida H
  • Journal Title: Obesity Volume: 21 Pages: 1625-1634
  • DOI: 10.1002/oby.20206
  • Peer Reviewed
• [Journal Article] Role of nitric oxide in muscle regeneration following eccentric muscle contractions in rat skeletal muscle (2013)
  • Author(s): Sakurai T, Kashimura O, Kano Y, Ohno H, Ji LL, Izawa T & Best TM
  • Journal Title: J Physiol Sci Volume: 63 Pages: 263-270
  • DOI: 10.1007/s12576-013-0262-y
  • Peer Reviewed
• [Journal Article] Skeletal muscle oxygenation during the nagewaza kakari exercise in Judo (2013)
  • Author(s): Haga S, Kizaki T, Sato S, Takemasa T, Ezaki K, Ueya K, Aita F, Hashimoto N, Ogasawara J, Sakurai T, Hamaoka T, Katsumura T, Sakurai T & Ohno H
  • Journal Title: Sport Sci Res Volume: 10 Pages: 233-241
  • URL: http://waseda-sport.jp/paper/1225/1225.pdf
  • Peer Reviewed
• [Journal Article] The Effects of Exercise Training on ObesityInduced Dysregulated Expression of Adipokines in White Adipose Tissue (2013)
  • Author(s): Sakurai T, Ogasawara J, Kizaki T, Sato S, Ishibashi Y, Takahashi M, Kobayashi O, Oh-Ishi S, Nagasawa J, Takahashi K, Ishida H, Izawa T & Ohno H
  • Journal Title: Int J Endocrinol Volume: 2013 Pages: 801743
  • DOI: 10.1155/2013/801743
  • Peer Reviewed
• [Journal Article] Effects of resistance exercise on intramuscular oxygenation and muscle fiber composition (2013)
  • Author(s): Aita F, Haga S, Sato S, Sakurai T, Esaki K, Hamaoka T, Mizuno M, Toshinai K, Miyazaki H, Takamasa T, Hashimoto N, Ogasawara J, Katsumura T, Kizaki T & Ohno H
  • Journal Title: J Sports Med Doping Stud Volume: 3 Pages: 3-9
  • DOI: 10.4172/2161-0673.1000133
  • Peer Reviewed
• [Journal Article] Exercise and oxidative stress in hypoxia (2013)
  • Author(s): Nagasawa J, Kizaki T & Ohno H
  • Journal Title: J Phys Fitness Sports Med Volume: 2 Pages: 481-486
  • DOI: 10.7600/jpfsm.2.481
  • Peer Reviewed
• [Journal Article] Association of smoking with intraocular pressure in middle-aged and older Japanese residents (2013)
  • Author(s): Yoshida M, Take S, Ishikawa M, Kokaze A, Karita K, Harada M & Ohno H
  • Journal Title: Environ Health Prev Med Volume: 19 Pages: 100-107
  • DOI: 10.1007/s12199-013-0359-1
  • Peer Reviewed
• [Journal Article] Interrelationship among blood pressure, intraocular pressure, and life-style in middle-aged and older Japanese residents (2013)
  • Author(s): Yoshida M, Take S, Ishikawa M, Karita K, Kokaze A, Harada M & Ohno H
  • Journal Title: Health Volume: 5 Pages: 1527-1532
  • DOI: 10.4236/health.2013.510207
  • Peer Reviewed
• [Journal Article] 高所環境は肥満を改善する (2013)
  • Author(s): 大野秀樹, 木崎節子, 櫻井拓也, 小笠原準悦, 佐藤章悟, 石橋義永, 宮澤伸子, 長澤純一, 野口いづみ, 貫田宗男, 荻原理江, 村上和子, 石田均, 宮崎裕美, 斎藤大蔵, 小林靖, 鈴木健二, 人見嘉哲, 中野法彦, 大河原知水, 大石修司, 桜井智野風, 井澤鉄也, 白土健, 今泉和彦, 芳賀脩光
  • Journal Title: 登山医学 Volume: 33 Pages: 167-171
  • Peer Reviewed
• [Presentation] 運動と脂肪細胞の働き (2014)
  • Author(s): 小笠原準悦, 櫻井拓也, 石橋義永, 木崎節子, 白土健, 今泉和彦, 井澤鉄也, 大野秀樹
  • Organizer: 第160回日本体力医学会関東地方会
  • Place of Presentation: 調布
  • Year and Date: 2014-03-08
• [Presentation] A circadian clock gene Rev-erb α modulates inflammatory functions of macrophages through negative regulation of monocyte chemoattractant protein-1 expression (2013)
  • Author(s): Kizaki T, Sato S & Ohno H
  • Organizer: 第42回日本免疫学会学術総会
  • Place of Presentation: 幕張
  • Year and Date: 2013-12-11
• [Presentation] 運動は骨格筋前駆細胞から褐色脂肪細胞への分化を誘導するか : 肥満予防・治療への応用 (2013)
  • Author(s): 小笠原準悦, 櫻井拓也, 木本紀代子, 木崎節子, 高橋和人, 住谷由計, 石田均, 大野秀樹
  • Organizer: 第42回杏林医学会総会
  • Place of Presentation: 三鷹
  • Year and Date: 2013-11-16
• [Presentation] 登山時の低酸素ストレス応答 (2013)
  • Author(s): 長澤純一, 野口いづみ, 笹尾真美, 佐藤章悟, 小笠原準悦, 櫻井拓也, 石橋義永, 木崎節子, 芳賀脩光, 大野秀樹
  • Organizer: 第68回日本体力医学会大会
  • Place of Presentation: 東京
  • Year and Date: 2013-09-22
• [Presentation] 時計遺伝子Rev-erbαはMCP1遺伝子の発現を抑制しマクロファージ免疫機能を制御する (2013)
  • Author(s): 佐藤章悟, 木崎節子, 櫻井拓也, 小笠原準悦, 石橋義永, 長澤純一, 桜井智野風, 井澤鉄也, 今泉和彦, 芳賀脩光, 大野秀樹
  • Organizer: 第68回日本体力医学会大会
  • Place of Presentation: 東京
  • Year and Date: 2013-09-21
• [Presentation] 水泳運動は前駆細胞からの褐色脂肪細胞化を促す (2013)
  • Author(s): 小笠原準悦, 櫻井拓也, 木崎節子, 佐藤章悟, 石橋義永, 井澤鉄也, 宮崎裕美, 斎藤大蔵, 十枝内厚次, 大石修司, 芳賀脩光, 大野秀樹
  • Organizer: 第68回日本体力医学会大会
  • Place of Presentation: 東京
  • Year and Date: 2013-09-21
• [Presentation] 脂肪組織および骨格筋組織における時計遺伝子と脂質代謝関連遺伝子の関連性 (2013)
  • Author(s): 加藤久詞, 増田慎也, 高倉久志, 佐藤章悟, 小笠原準悦, 櫻井拓也, 木崎節子, 桜井智野風, 大野秀樹, 井澤鉄也
  • Organizer: 第68回日本体力医学会大会
  • Place of Presentation: 東京
  • Year and Date: 2013-09-21
• [Presentation] Effect of frequent antioxidant supplementation on oxidative stress when climbing Mt Fuji (2013)
  • Author(s): Nagasawa J, Noguchi I, Sasao M, Sato S, Sakurai T, Ogasawara J, Ishibashi Y, Kizaki T & Ohno H
  • Organizer: 21st International Congress on Nutrition and Integrative Medicine
  • Place of Presentation: Sapporo
  • Year and Date: 2013-07-28
• [Presentation] Inhibitory effect of ETAS against amyloid beta-induced cellular disorder in PC12 cells (2013)
  • Author(s): Ogasawara J, Ito T, Takanari J, Sakurai T, Kizaki T, Sato S, Ishibashi Y, Nagasawa J, Saito D, Nakano N, Izawa T & Ohno H
  • Organizer: 21st International Congress on Nutrition and Integrative Medicine
  • Place of Presentation: Sapporo
  • Year and Date: 2013-07-27
• [Presentation] 運動は肥満による脂肪組織の線維化関連因子の発現増加を減弱させる (2013)
  • Author(s): 櫻井拓也, 小笠原準悦, 木崎節子, 長澤純一, 芳賀脩光, 大野秀樹
  • Organizer: 第21回日本運動生理学会大会
  • Place of Presentation: 川越
  • Year and Date: 2013-07-27
• [Presentation] 持久性走トレーニングによる白色脂肪細胞の脂肪分解反応の亢進にはATGLの発現変化が関与する (2013)
  • Author(s): 小笠原準悦, 櫻井拓也, 佐藤章悟, 石橋義永, 長澤純一, 大石修司, 芳賀脩光, 井澤鉄也, 木崎節子, 大野秀樹
  • Organizer: 第158回日本体力医学会関東地方会
  • Place of Presentation: 新座
  • Year and Date: 2013-07-13
• [Book] 身体の活性酸素消去を高める方法.In : 活性酸素の本当の姿, 鈴木敬一郎編 (2014)
  • Author(s): 大野秀樹, 木崎節子
  • Total Pages: 149-169
  • Publisher: ナップ
• [Book] ここまでわかった燃える褐色脂肪組織の不思議 (2013)
  • Author(s): 斎藤昌之, 大野秀樹編
  • Total Pages: 158
  • Publisher: ナップ
• [Book] 酵素処理アスパラガス茎熱水抽出物はアミロイドαにより誘導されるPC12細胞の細胞障害を抑制する.In : 第21回統合医療機能性食品国際会議議事録 (2013)
  • Author(s): 小笠原準悦, 伊藤知洋, 高成準, 櫻井拓也, 木崎節子, 佐藤章悟, 石橋義永, 長澤純一, 斎藤大蔵, 中野法彦, 井澤鉄也, 大野秀樹
  • Total Pages: 51-56
  • Publisher: AHCC研究会
• [Book] 富士登山時の酸化ストレスに対する抗酸化剤頻回摂取の効果.In : 第21回統合医療機能性食品国際会議議事録 (2013)
  • Author(s): 長澤純一, 野口いづみ, 笹尾真美, 佐藤章悟, 櫻井拓也, 小笠原準悦, 石橋義永, 木崎節子, 大野秀樹
  • Total Pages: 83-87
  • Publisher: AHCC 研究会