2015 Fiscal Year Final Research Report
Microfluidic device to culture an early-stage mouse embryo for morphogenesis studies
| Project/Area Number |
25600060
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nano/Microsystems
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| Research Institution | Kyoto University |
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Principal Investigator |
Yokokawa Ryuji 京都大学, 工学(系)研究科(研究院), 准教授 (10411216)
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| Co-Investigator(Renkei-kenkyūsha) |
MIURA Takashi 九州大学, 大学院医学研究院, 教授 (10324617)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | マイクロ・ナノデバイス / 細胞・組織 / 自己組織化 / 生体機能利用 / マイクロフルイディクス / MicroTAS / MEMS |
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| Outline of Final Research Achievements |
The purpose of this study was to develop a microfluidic system that provides a three-dimensional cell culture environment by the reconstituted vascularized networks. It enables long-term tissue culture on a chip for morphogenesis studies. We have developed an on-chip angiogenesis assay, optimized spheroid culture condition to be injected to the chip, and realized vascularized network with a spheroid embedded. Further, the network was found perfusable by injecting microspheres and fluorescent dyes.
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| Free Research Field |
BioMEMS，MicroTAS
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