Evolutionary stability and developmental dynamics of the plant mitochondrial genome

2015 Fiscal Year Final Research Report

• Project/Area Number: 25640102
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Kobe University
• Principal Investigator: Mori Naoki 神戸大学, (連合)農学研究科(研究院), 教授 (60230075)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: コムギ / ミトコンドリアゲノム / 進化的安定性 / 分子内組換え / 細胞質置換系統 / Aegilops caudata / マイクロサテライト

Outline of Final Research Achievements

Mitochondria and chloroplast are deeply involved in the important activities in the cell through the complex interaction between nuclear genome. To examine the evolutionary stability of the plasmon, we evaluated the molecular variation in chloroplast and mitochondrial genome between the alloplasmic wheat line with an alien cytoplasm and the donor line of the cytoplasm. This alloplasmic line has been maintained for more than 60 generation by repeated backcrossing. No variation was identified between the alloplasmic line and the donor line, indicated the evolutionary stable nature of the plasmon in wheat. In addition, we found that the intramolecular recombination in mitochondrial genome occurs in almost all of the tissues, although the frequency is very low as well as variable among tissues.

Free Research Field

植物遺伝学