Structural Characterization of Subunit-Specific Isotope labelled Membrane Bound Protein

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650016
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Hokkaido University
• Principal Investigator: ISHIMORI Koichiro 北海道大学, 理学(系)研究科(研究院), 教授 (20192487)
• Co-Investigator(Kenkyū-buntansha): UCHIDA Takeshi 北海道大学, 大学院理学研究院, 准教授 (30343742)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: シトクロム酸化酵素 / ナノディスク / 呼吸鎖電子伝達系 / NMR / MSP

Outline of Final Research Achievements

To discuss the electron transfer mechanism in the respiratory chain in mitochondria, the reconstitution of an isotope labelled key enzyme, cytochrome c oxidase, into the nano-disc, a mimic of membrane in cells, has been examined, which allows us to measure the high resolution NMR spectra of the membrane-bound proteins under the physiological condition. The expression, purification and reconstitution of the bacterial cytochrome c oxidase into the nano-disc were confirmed, leading to the high resolution structural analysis of high molecular weight membrane bound proteins.

Free Research Field

生物無機化学