Establishment of an over-expression system for mammalian large membrane proteins

2016 Fiscal Year Final Research Report

• Project/Area Number: 25650020
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Ogawa Haruo 東京大学, 分子細胞生物学研究所, 准教授 (40292726)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 大量発現 / 膜蛋白質 / アデノウィルス / 超巨大蛋白質

Outline of Final Research Achievements

Although the number of atomic structures of membrane proteins deposited in PDB increases year by year, most of them are bacterial homologues of mammalian ones or low molecular-weight ones such as GPCRs. X-ray crystallographic studies of mammalian large membrane proteins have not been conducted well. One of the reasons is that there is no easy as well as versatile system for the protein production of such proteins. Thus, we aimed to establish an over-expression system for mammalian large membrane proteins, such as IP3 receptor, with modifications of our own expression system. The new system should be useful for carrying out kinetic studies for such proteins which could not be studied so far. Moreover, high-resolution strucuture of such proteins should be useful for new drug-development.

Free Research Field

構造生物学