Development of novel metabolic engineering technology using one-carbon compounds and their metabolic regulatory systems

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660079
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: YURIMOTO Hiroya 京都大学, (連合)農学研究科(研究院), 准教授 (00283648)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ホルムアルデヒド / メタノール / 遺伝子発現

Outline of Final Research Achievements

In order to develop novel metabolic engineering technology using one-carbon (C1) compounds and their metabolic regulatory systems, we investigated the metabolic flow of gene-engineered strains harboring the artificial bifunctional formaldehyde-fixation enzyme and molecular function of transcriptional regulators involved in regulation of C1 metabolism. Furthermore, we found that the sensor proteins localized in plasma membrane were involved in regulation of methanol-inducible gene expression in the methylotrophic yeast.

Free Research Field

応用微生物学