Functions and mechanisms of ascorbic acid in maintaining hepatic cytochrome P-450

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660102
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Nagoya University
• Principal Investigator: Horio Fumihiko 名古屋大学, 生命農学研究科, 教授 (20165591)
• Co-Investigator(Renkei-kenkyūsha): Murai Atsushi 名古屋大学, 大学院生命農学研究科, 准教授 (10313975) ; Kobayashi Misato 名古屋大学, 大学院生命農学研究科, 助教 (20456586)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: アスコルビン酸 / アスコルビン酸欠乏 / シトクロムP-450 / ヘム分解 / ヘムオキシゲナーゼ

Outline of Final Research Achievements

The mechanisms underlying the decrease in hepatic cytochrome P-450 (CYP) content in ascorbic acid deficiency was investigated in scurvy-prone ODS rats. Firstly, ascorbic acid deficiency decreased hepatic microsomal total CYP content, CYP2B1/2B2 protein, and mitochondrial cytochrome oxidase (COX) complex IV subunit I protein, and simultaneously increased heme oxygenase-1 protein in microsomes and mitochondria. Nextly, heme oxygenase-1 inducers, that is lipopolysaccharide and hemin, were administered to phenobaribital-treated ODS rats fed sufficient ascorbic acid. The administration of these inducers decreased hepatic microsomal total CYP content, CYP2B1/2B2 protein, and mitochondrial COX complex IV subunit I protein. These results suggested that the stimulation of hepatic heme oxygenase-1 expression by ascorbic acid deficiency caused the decrease in CYP content in liver.

Free Research Field

栄養生化学