Optimization of direct reprograming methods by understanding the epigenomic features of descendant cell types.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660256
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: Keio University
• Principal Investigator: ODA Mayumi 慶應義塾大学, 医学部, 助教 (80567511)
• Research Collaborator: SAKOTA Miki 慶應義塾大学, 医学部, 研究員
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 直接リプログラミング / DNAメチル化 / 転写因子 / ES細胞 / エピゲノム

Outline of Final Research Achievements

In this study, we assessed the optimization of direct reprogramming method by analyzing the expression data of transcription factor (TF)-induced ES cell lines and found that the efficiency of cell differentiation examined by several cell type-specific markers was changed according to the role of TFs in direct reprogramming process. We also examined different culture conditions that alters genome-wide epigenetic status, and studied the results of TF-induction under different DNA methylation status. For the comparison, DNA methylation profiles of several tissues were made, which is expected to be used for the understanding the cell type-specific epigenomic patterns and the differences between their descendants.

Free Research Field

エピゲノム学