Assembly of baculovirus genome DNA fragments in yeast for improving baculovectors

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660260
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Insect science
• Research Institution: Hokkaido University
• Principal Investigator: BANDO Hisanori 北海道大学, (連合)農学研究科(研究院), 教授 (20189731)
• Co-Investigator(Kenkyū-buntansha): SATO Masanao 基礎生物学研究所, 発生生物学領域, 助教 (20517693)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 昆虫機能利用 / 有用物質生産

Outline of Final Research Achievements

The technologies that enable flexible manipulation of virus genome are very important technical base for improving viral vectors. The aim of this study is to establish a method for assembling baculovirus genome DNA from both synthetic and natural DNA fragments to improve baculovectors. First, we constructed a baculovirus of the silkworm (BmNPV/bacmid) carrying an yeast replication origin (Yori). Second, we cloned 21 overlapping DNA fragments covering whole sequence of the BmNPV/bacmid genome DNA with Yori in bacterial cells using a plasmid vector. Lastly, we assembled the 21 fragments in yeast to form the complete BmNPV genome DNA.

Free Research Field

応用分子昆虫学