2014 Fiscal Year Final Research Report
An interaction elucidation and its application of endobacteria present inside the hyphae of endophyte
| Project/Area Number |
25660273
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Hiroyuki 茨城大学, 農学部, 教授 (80168947)
SATO Yoshinori 独立行政法人国立文化財機構東京文化財研究所, 保存修復科学センター, 研究員 (50466645)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 共生 / 菌類内生バクテリア / エンドファイト |
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| Outline of Final Research Achievements |
Microscopic and molecular analyses showed the presence of endobacteria inside the hyphae of randomly selected fungal species. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal isolates and most of the sequences were related to the family Burkholderiaceae.
Microscopic observation confirmed the presence of rod-shaped, motile bacteria living inside and outside the hyphae of Veronaeopsis simplex all isolates. The PCR results using universal primers for 16S sRNA positively detected the presence of bacteria on V. simplex. Analysis of terminal-restriction fragment length polymorphism (T-RFLP) coupled with the sequence data from 16S rDNA gene cloning revealed that Rhizobium sp. was found to be associated with the fungal isolates. In conclusion the findings suggest that V. simplex has a complex interaction with associated bacteria involving host plant growth promotion effect.
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| Free Research Field |
農学
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