Retrieval of blood-brain barrier transport function towards dementia prevention

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670067
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: Tachikawa Masanori 東北大学, 薬学研究科(研究院), 准教授 (00401810)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 血液脳関門 / 認知症 / トランスポーター / 輸送タンパク質 / 定量プロテオミクス / チャネル

Outline of Final Research Achievements

The purpose of the present research was to clarify the blood-brain barrier (BBB) transport system for dementia-preventing substances and the changes in transport functions and transporter expression levels under brain vascular disease conditions. A transport protein of docosahexaenoic acid (DHA) was identified in the human brain endothelial cells (hCMEC/D3 cells). The DHA uptake by hCMEC/D3 cells was enhanced by an activating factor. In vitro study with the transwell culture of hCMEC/D3 cells indicated that ischemia-related changes in the BBB transport involved the basal channel opening. Quantitative targeted absolute proteomics revealed the changes in the expression profiles of transporter proteins at the BBB in mouse models of brain vascular disorders. These results could provide the basis of dementia prevention by the retrieval of blood-brain barrier transport function.

Free Research Field

神経薬剤学、定量プロテオミクス