Trial of generating HIV-1-susceptible rhesus monkeys by using technologies in reproductive medicine

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670218
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: Shioda Tatsuo 大阪大学, 微生物病研究所, 教授 (00187329)
• Co-Investigator(Kenkyū-buntansha): AKARI Hirofumi 京都大学, 霊長類研究所, 教授 (20294671)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: HIV感染症 / iPS細胞 / 再生医療 / アカゲザル

Outline of Final Research Achievements

Currently it is impossible to eradicate HIV from infected individuals since HIV persists in resting memory T cells that have long half-life. Therefore, it is necessary to work out a totally novel strategy to eradicate HIV from infected individuals. To apply iPS cell technologies to HIV eradication strategies, we established iPS cells from peripheral blood mononuclear cells of rhesus monkeys. Human Oct3/4, Sox2, Klf4, and c-Myc genes worked in rhesus monkey cells for reprograming, although more stringent condition was required to establish rhesus monkey iPS cells. We were able to induce CD34+ hematopoietic stem cells and macrophage-like cells from the rhesus monkey iPS cells. We are now planning autologous transplantation of iPS cells with a marker into rhesus monkeys.

Free Research Field

ウイルス学