2014 Fiscal Year Final Research Report
Development of local microRNA delivery system and in vivo application
Project/Area Number |
25670383
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Cardiovascular medicine
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Research Institution | Kyoto University |
Principal Investigator |
ONO KOH 京都大学, 医学(系)研究科(研究院), 准教授 (00359275)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Keywords | マイクロRNA / ステント / ナノ粒子 / 動脈硬化 |
Outline of Final Research Achievements |
Recently, drug eluting stent (DES) is mainly used for the treatment of coronary artery diseases. However, the drug can inhibit the growth of endothelial cells, which causes thrombosis or chronic inflammation. Therefore, the ideal treatment is to regenerate the endothelial cells and inhibit the growth of smooth muscle cells. We utilized miR-126 which can induce the regeneration of endothelial cells and inhibit inflammation for this experiment. We generated cholesterol conjugated double strand synthetic RNAs which can overexpress mature miR-126 locally without any vectors. We loaded there double strand RNAs into poly lactic-co-glycolic acid (PLGA) nano-particles and coated the stent with these PLGA nano-particles. We evaluated the effect of this stent in rabbit model of vascular injury. miR-126-eluting stent could significantly inhibit the restenosis of iliac artery after vascular injury compared with control RNA-eluting stent.
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Free Research Field |
循環器内科学
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