Elucidation of the regulation of expression of receptors for incretin and PACAP in pancreatic beta cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670435
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: Yokohama City University
• Principal Investigator: TERAUCHI Yasuo 横浜市立大学, 医学(系)研究科(研究院), 教授 (40359609)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 膵β細胞 / インクレチン

Outline of Final Research Achievements

We investigated the effects of glucose and AMPK in the regulation of incretin receptors expression in pancreatic islets. The expression of incretin receptors in bell-shaped response to glucose; Expression of the incretin receptors in the isolated islets showed higher levels under a medium glucose concentration (11.1 mM) than that under a low glucose concentration (2.8 mM), but was suppressed under a high glucose concentration (22.2 mM). The phosphorylation of AMPK in the mouse islets was decreased by increasing glucose concentrations. Both treatment with an AMPK inhibitor and DN-AMPK expression produced a significant increase of the incretin receptors expression under a low glucose concentration. Taken together, AMPK is involved in the regulation of incretin receptors expression in pancreatic islets under a low glucose concentration.

Free Research Field

糖尿病学