Promoting differentiation of osteoblasts from induced pluripotent stem (iPS) cells using scaffolds recreating the bone tissue microenvironment.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670859
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: MIYAMOTO Youji 徳島大学, ヘルスバイオサイエンス研究部, 教授 (20200214)
• Co-Investigator(Kenkyū-buntansha): NAGAI Hirokazu 徳島大学, 大学院ヘルスバイオサイエンス研究部, 准教授 (50282190) ; TAMATANI Tetsuya 徳島大学, 病院, 講師 (30274236) ; UCHIDA Daisuke 獨協医科大学, 医学部, 准教授 (20335798)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 再生医学 / iPS細胞 / 骨再生

Outline of Final Research Achievements

In this study, we try to promote osteoblastic differentiation of human induced pluripotent stem (iPS) cells using scaffolds recreating the bone tissue microenvironment.
As a result, it was revealed that 1) carbonate apatite (CAp) foam was fabricated based on compositional conversion reaction using α-tricalcium phosphate foam as a precursor, 2) low-crystalline CAp foam with 93%～96% porosity and fully interconnected porous structure was fabricated, 3) the differentiation of human iPS cells to mesenchymal stem cell-like cells was induced, 4) CAp was resorbed by osteoclast in vivo, 5) mesenchymal stem cells induced from iPS cells cultured on carbonate apatite differentiated into osteoblastic cells, 6) CAp granules combined with BMP-2 stimulated the ectopic new bone formation by promoting osteoblastic differentiation of mesenchymal cells in the surrounding tissue.

Free Research Field

医歯薬学