Development the system controlling cell function based on the gene regulation via the ubiquitin proteasome pathway

2015 Fiscal Year Final Research Report

• Project/Area Number: 25702028
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyoto University
• Principal Investigator: HIGUCHI Yuriko 京都大学, 健康長寿社会の総合医療開発ユニット, 講師 (40402797)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ユビキチンプロテアソーム経路 / 遺伝子発現制御 / 細胞製剤

Outline of Final Research Achievements

The gene regulation in the cell would lead a novel DDS in the cell based therapy. The purpose of this study is to develop the gene regulation system which responses to the microenvironment in the body. The repressor fused with IkBa was constructed and co-transfected with the vector expressing beta-galactosidase as a reporter protein which was inserted downstream of the operator. Beta-galactosidase expression was induced in those cells by TNFa treatment. We designed the repressor which is degraded via the ubiquitin proteasome pathway and developed a novel gene regulation system which activated by TNFa treatment

Free Research Field

ドラッグデリバリー