2016 Fiscal Year Final Research Report
Development of versatile N-oxide-based fluorescent probes to study pathological dysfunction of iron metabolism
| Project/Area Number |
25702050
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Research Collaborator |
NIWA Masato
TSUBOI Hitomi
KADOTA Satoki
IKESHITA Yukie
MIKI Ayaji
KATABE Tomoyo
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 鉄 / イメージング / 蛍光プローブ |
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| Outline of Final Research Achievements |
We have developed a series of fluorescent probes for Fe(II) ion, which is an important chemical species in the iron metabolism, to study biological and pathological roles of iron ion in living cells. The design concept of Fe(II) fluorescent probe is based on N-oxide chemistry, where Fe(II) ion selectively reduces N-oxide by deoxygenation. In this research, we have succeeded in development of a color variation of the probes ranging from blue to deep-red by utilizing N-oxide chemistry as a selective fluorogenic molecular switch. We have also developed a membrane-anchoring fluorescent probe to visualize release of Fe(II) ion during endocytotic uptake of iron induced by transferrin/transferrin receptor interaction.
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| Free Research Field |
ケミカルバイオロジー
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