Nanoparticle Assembly mediated by Designed DNA Nanostructures

2016 Fiscal Year Final Research Report

• Project/Area Number: 25706009
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Nanobioscience
• Research Institution: Nagoya University
• Principal Investigator: Tagawa Miho 名古屋大学, 未来材料・システム研究所, 准教授 (40512330)
• Research Collaborator: GANG Oleg Brookhaven National Lab. ; Yager Kevin Brookhaven National Lab. ; Starr Francis Wesleyan Univ.
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: DNA / ナノ粒子 / self-assembly / 自己集合 / 超格子構造 / コロイド結晶

Outline of Final Research Achievements

We demonstrated the formation of lattice using DNA-functionalized nanoparticles (DNA-NPs) linked by DNA tetrahedral constructs. DNA tetrahedron has four symmetrical connecting bonds and plays a determining role in the inter-particle bonding; that allows for control of particles coordination. Using small angle x-ray scattering analysis, we observed and revealed the assembly of DNA-NPs into a diamond structure.
We also performed two-dimensional programmable assembly of nanoparticles on supported lipid bilayers (SLBs). Owing the high mobility of lipid molecules, two-dimensional diffusion of DNA-NPs on SLB was achieved. Using AFM imaging, we studied the morphology of the formation of DNA-NPs on SLB and its changes with annealing. We observed that DNA-NPs assemble into a densely-packed single-layered two-dimensional lattice through DNA hybridization.

Free Research Field

ナノバイオサイエンス