Elucidation of the mechanisms of skeletal muscle mitochondrial biogenesis by lactate

2016 Fiscal Year Final Research Report

• Project/Area Number: 25750331
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: The University of Tokyo
• Principal Investigator: Hoshino Daisuke 東京大学, 大学院理学系研究科(理学部), 特任助教 (70612117)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 乳酸 / ミトコンドリア / 運動 / 転写制御 / 骨格筋

Outline of Final Research Achievements

We examined whether lactate causes skeletal muscle mitochondrial biogenesis, using extracted mouse muscle incubation system and electric stimulation system to C2C12 myotubes. As a result, 24h-lactate stimulation (10 mM) for extracted mouse muscle suppressed mRNA contents of PGC-1alpha and mitochondria. However lactate concentration exceeded 25 mM after 24 hours of lactate incubation, which was non-physiological high lactate concentration. Elevation in lactate concentration due to electrical stimulation increased the mRNA contents of PGC-1alpha in C2C12 myotubes. These results suggest that the increase in lactate concentration associated with muscle contraction may cause mitochondrial biogenesis via transcriptional regulation. It is necessary to clarify the dose response of lactate concentration in further studies.

Free Research Field

運動生化学