2015 Fiscal Year Final Research Report
Creation of artificial functional lectins for target-selective labeling of carbohydrates and their application to cell surface engineering
| Project/Area Number |
25750387
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 糖鎖認識 / レクチン / ラベル化 / 人工機能性タンパク / 固相反応 / 光反応 |
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| Outline of Final Research Achievements |
Toward the creation of novel artificial functional lectins, a solid-phase affinity labeling method (1st generation) using a designed and synthesized chemical tool was developed. It was found that the solid-phase affinity labeling reaction of target-lectin (Con A or PNA) selectively and effectively proceeded even in the presence of other proteins. In addition, it was successfully demonstrated that target-selective isolation and modification of the native protein, hCA II, was feasible, even from human cell lysate. With these results, it is expected that this method will be applicable for target-selective isolation and modification of unknown target receptor proteins of biologically active small molecules. Furthermore, a novel solid-phase affinity labeling method (2nd generation) using a designed and synthesized photo-cleavable chemical tool to improve the efficiency of the cleavage reaction. These results will contribute to the creation of the purposed artificial functional lectins.
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| Free Research Field |
有機合成化学、糖質化学、化学生物学
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