Analysis of the physiological roles and dynamics of TGN in plant cell

2015 Fiscal Year Final Research Report

• Project/Area Number: 25840100
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The University of Tokyo
• Principal Investigator: Uemura Tomohiro 東京大学, 理学(系)研究科(研究院), 助教 (90415092)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: オルガネラ / ＴＧＮ / ライブイメージング

Outline of Final Research Achievements

The trans-Golgi network (TGN) functions as a sorting station that directs cargo proteins to a variety of destinations including post-Golgi compartments and the extracellular space. However, the functions and dynamics of the TGN in plant cells have remained unknown. To reveal the dynamics of the plant TGN, transgenic plants expressing GFP-SYP43 as a TGN marker was observed. Observations revealed that there are two types of TGN in Arabidopsis root: the GA-TGNs (Golgi-associated TGNs), located on the trans-side of the Golgi apparatus, and the GI-TGNs (Golgi-released independent TGNs), located away from the Golgi apparatus and behaving independently. The GI-TGNs is segregated from the GA-TGNs. I also found that the number of the GI-TGNs are different among observed tissues.

Free Research Field

植物細胞生物学