2014 Fiscal Year Final Research Report
Examination of gem one editing for effective plant breeding
| Project/Area Number |
25850004
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 遺伝子導入 / 変異作出 |
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| Outline of Final Research Achievements |
In the plant breeding by mutation, it is necessary to identify new interest plant cultivars for a long period, because the phenotype of interest was selected from various mutants caused by the radiation. To shorten the period, it is required that exact mutation can induce on targeted gene only. In this study, we examined to apply TALEN and CRISPR/Cas9 system to a new method of breeding plant in tomato and strawberry. As a result, the mutations that loss of the 1-3 bases on the target genome region were observed in tomato calli by using TALEN or CRISPR/Cas9 system. In addition, MYB10 and MYB1 were involved in coloration of receptacle by Agrobacterium-mediated RNAi in strawberry.
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| Free Research Field |
農学
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