Establishment of cell transplantation using stem cell and olfactory ensheathing cell into dog with spinal cord injury

2015 Fiscal Year Final Research Report

• Project/Area Number: 25850212
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Nihon University
• Principal Investigator: ITO Daisuke 日本大学, 生物資源科学部, 講師 (40508694)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 脊髄再生 / 再生医療 / 椎間板ヘルニア / 脱分化脂肪細胞 / DFAT / 鼻粘膜

Outline of Final Research Achievements

The purpose of this study is to investigate the possibility of stem cell and olfactory ensheathing cell transplantation to naturally occurred spinal cord injury in dogs.To establish the methods, it was analized whether stem cells and olfactory ensheathing could be harvested from canine nasal mucosa of which were obtained via nasal cavity using endoscopy. Some cells expressed stem cell markers on nasal mucosa section, and other cells expressed olfactory ensheathing cell marker. In addtion, those cells could be cultured. However infection were seen in few cases. Therefore further methods would be needed for this study from safety.
To find altanative cell source, de-defferentiated fat cells (DFAT),which are guaranteed the safety,were transplanted to spinal cord injury model mice to analyze the efficacy and safety. DFAT transplanted mice showed significantly better walking steps than control mice.

Free Research Field

細胞移植による脊髄再生医療