Development of treatment technology for bovine mastitis by recombinant bovine cytokine intramammary infusion at dry-off.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25850215
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: KIKU Yoshio 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門病態研究領域, 上級研究員 (70370179)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 乳房炎 / 牛 / サイトカイン / 治療 / 予防

Outline of Final Research Achievements

The objective of this study was to investigate the effect for treatment or prevention of bovine mastitis of the intramammary infusion of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) produced in transgenic silkworm as a dry cow therapy. As the treatment or preventive trials, the infected or non-infected quarter were assigned at drying-off to an intramammary infusion of rbGM-CSF (400 μg/quarter) solutions. Each milk sample was collected for bacteriological examination and somatic cell count (SCC) at dry-off and a month after calving. In the treatment trial, total bacterial counts and SCC in milk was lower at 0, 7, 14 and 28 days after calving than at dry-off. In the prevention trial, the new intramammary infection of all cows was not detected after calving. These results suggest that the intramammary infusion of rbGM-CSF at drying off has a high potential as an agent of dry cow therapy.

Free Research Field

臨床獣医学、獣医免疫学