Transient Tcf3 Gene Repression by TALE-Transcription Factor Targeting

2015 Fiscal Year Final Research Report

• Project/Area Number: 25860797
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Okayama University
• Principal Investigator: Masuda Junko 岡山大学, 自然科学研究科, 助教 (20424674)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: TCF3 (E2A) / 人工転写因子 / TALE

Outline of Final Research Achievements

Transplantation of hematopoietic stem and progenitor cells (HSCs) is now a widely performed therapy for many hematopoietic diseases. However, these cells are present in low number and are subject to replicative senescence after extraction; thus, the acquisition of sufficient numbers of cells for transplantation requires donors able to provide repetitive blood samples and/or methods of expanding cell numbers without disturbing cell multipotentiality. Previous studies have shown that HSCs maintain their multipotentiality and self-renewal activity if TCF3 transcription function is blocked under B cell differentiating conditions. Taking advantage of this finding to devise a new approach to HSC expansion in vitro, we constructed an episomal expression vector that specifically targets and transiently represses the TCF3 gene.

Free Research Field

血液内科学