Organ reengineering of a small bowel graft using decellularized scaffold of porcine intestine and intestinal organoid

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861164
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Keio University
• Principal Investigator: Hibi Taizo 慶應義塾大学, 医学部, 講師 (10338072)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 移植外科学 / 小腸グラフト

Outline of Final Research Achievements

We aimed to develop a transplantable small bowel graft with the organ decellularization technique using porcine intestinal scaffold and intestinal organoid as a future treatment alternative for intestinal failure patients waiting for small bowel transplantation. After the decellularization technique of porcine intestine matured, we switched the plan to use rat intestine because of logistic reasons. We successfully reperfused the small bowel graft through the vessels with heparinized saline and confirmed that there is no cell nuclei within the scaffold by immunohistochemical staining. We are currently conducting precise observation of the internal structure of the graft using electron microscope and evaluation of DNA included in the cytoskeleton and the nature of extracellular matrix.

Free Research Field

肝胆膵・移植外科