Analysis of angiogenesis for induced-gut from pluripotent stem cells : for gut transplantation without rejection

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861211
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Nara Medical University
• Principal Investigator: UEDA Takeshi 奈良県立医科大学, 医学部附属病院, 研究員 (40526810)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 多能性幹細胞 / 腸管分化誘導 / iPS細胞 / 脂肪由来幹細胞 / 短腸症候群

Outline of Final Research Achievements

We tried to perform the long-term culture in vitro with our gut-like organ differentiation from induced pluripotent stem (iPS) cells. We paid attention to adipose tissue-derived stem cells (ADSC) with ability for angiogenesis, we cultured iPS cells and ADSCs together.
We tried to differentiation of intestinal mesentery, we demonstrated that ADSCs expressed the vascular endothelial marker genes such as CD31. However we could not develop fatty tissue with vascular formation. We tried to angiogenesis for gut-like organ using the embryoid body from iPS cells and ADSCs by three-demensional culture. We could not confirm the vascular formation. We could not demonstrate the angiogenesis with vascular formation for gut-like organ in vitro.

Free Research Field

消化器外科