Mechanisims of isoflurane-induced carrdiac protection from ischemia/reperfusion injury: a role for glucagon-like peptide-1.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861377
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology
• Research Institution: The University of Tokushima
• Principal Investigator: KAWANO Hiroaki 徳島大学, ヘルスバイオサイエンス研究部, 徳島大学特別研究員 (90467811)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: イソフルラン / グルカゴン様ペプチドー１ / プレコンディショニング / 心筋保護作用

Outline of Final Research Achievements

To assess the role of volatile anesthetic (isoflurane) and glucagon-like peptide-1 (GLP-1) in the protection from ischemia/reperfusion injury, we tested mice with isoflurane or exendine-4, GLP-1 agonist, and then exposed the mice to ischemia/reperfusion. Similar infarct size reductions were produced by treatment with isoflurane or exendine-4. The cardioprotective effects of these pharmacological preconditioning were abolished in GLP-1 antagonist pretreatment. Furthermore, apoptosis has been evaluated in these preconditionings and we found after 2 hours of reperfusion that apoptosis in myocardium at risk of ischemia is attenuated by preconditionings.
Additionally, to determine the downstream mediators of involved in isoflurane or GLP-1 preconditioning, we performed immunoblotting and realtime PCR using preconditioned samples.

Free Research Field

麻酔科学