2015 Fiscal Year Final Research Report
Localization and gene expression analysis of angiogenic factors in experimental periapical lesions
| Project/Area Number |
25861795
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Conservative dentistry
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 血管新生 / 根尖性歯周炎 / NF-κB |
|---|
| Outline of Final Research Achievements |
This study aimed to examine the involvement of angiogenetic factors in the development of experimental apical periodontitis in rat molars. Induction of apical periodontitis under the administration of an NF-kB inhibitor (NF-kB decoy) resulted in the reduction of (1) periapical radiolucent area, (2) CD31-positive endothelial cell density, and (3) mRNA expression levels of VEGFR2, CXCL1 and CXCR2.When rat endothelial cells and mesenchymal stem cells were co-cultured in the presence of LPS, addition of NF-kB decoy resulted in the downregulation of CXCL1 and CXCR2 mRNA expression in these cells and a significant decrease of VEGF in the culture medium. These results suggest that NF-kB signaling pathways in endothelial cells play a role in the pathogenesis of apical periodontitis.
|
| Free Research Field |
保存治療系歯学
|
